Aptamer-functionalized graphene-gold nanocomposites for label-free detection of dielectrophoretic-enriched neuropeptide Y
نویسندگان
چکیده
Article history: Received 19 May 2016 Received in revised form 31 July 2016 Accepted 15 September 2016 Available online 16 September 2016 We present an electrokinetically enhanced aptamer sensing platform on a disposable plastic chip for label-free detection of neuropeptide Y (NPY), which is a key neurological biomarker. The sensor consists of aptamerfunctionalized graphene-gold nanocomposites (Gr-AuNs) patterned inside a nanoslit that is embossed on cyclic olefin copolymer via nanoimprint lithography. Analyte molecules are dielectrophoretically focused through the nanoslit onto aptamer-immobilized Gr-AuNs for rapid and selective electrochemical detection of NPY at picomolar levels. © 2016 Elsevier B.V. All rights reserved.
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